| Enrofloxacin

Enrofloxacin, also known as ethyl ciprofloxacin or enrofloxacin, is a slightly yellow or pale yellow crystalline powder with a bitter taste. It is insoluble in water and is designated as a veterinary-specific drug by the state.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	25	12.5	6.25	3.12	1.56	0.78	0.39	0
OD	0.1	0.25	0.51	0.82	1.17	1.72	2.2	2.49

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Enrofloxacin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	82-105	96
Plasma(n=8)	87-98	93
Cell Culture Supernates(n=8)	83-114	96

 

| Linearity

Samples were spiked with high concentrations of Enrofloxacin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	100-114	86-104	99-108
	Average (%)	105	97	104
1 : 4	Range (%)	82-105	96-114	84-112
	Average (%)	98	103	103